Product Overview
Cells were isolated from an adult human dermal cell sample and programmed using our patented virus-free method using episomal DNA and our proprietary mix of vectors, excluding l-Myc, c-Myc, and Lin28 transcription factors.
The cell line was validated for pluripotency based on colony morphology, alkaline phosphatase expression, and expression of SSEA-4. Cells are free of Mycoplasma and exhibit classical morphology and growth characteristics.
Fibroblast cells can be differentiated into neural and epithelial progenitor cells for further use in cell therapy, regenerative medicine, wound healing, and tissue engineering research applications.
Vial contains approximately 500,000 cells. Shipped with dry ice.
Stability & Storage
| | |
Human Foreskin Fibroblast iPS Cells | -80°C
(preferably in the vapor phase of a liquid nitrogen storage unit) | |
Human iPS Cells Growth Media
(not included) | | |
complete media
(see Media Formulation Instructions on documentation) | | |
Avoid repeated freeze-thaw cycles for cells. Avoid repeated exposure to room temperature and light for media.
Publications
Development of a High-Efficacy Reprogramming Method for Generating Human Induced Pluripotent Stem (iPS) Cells from Pathologic and Senescent Somatic Cells
Tanaka, N et al. | International Journal of Molecular Sciences 2020 SEP
Department of Cardiology, Saitama Medical University
Department of Preventive Medicine and Public Health, Keio University School of Medicine.
Fibroblasts-dependent invasion of podoplanin-positive cancer stem cells in squamous cell carcinoma
Miyashita, T et al. | Journal of Cellular Physiology 2020 FEB
Department of Integrated Biosciences, Laboratory of Cancer Biology, The University of Tokyo.
Spatiotemporal characteristics of fibroblasts-dependent cancer cell invasion
Miyashita, T et al. | Journal of Cancer Research and Clinical Oncology 2018 NOV
Department of Integrated Biosciences, Laboratory of Cancer Biology, The University of Tokyo.
Cell Injury-Induced Release of Fibroblast Growth Factor 2: Relevance to Intracerebral Mesenchymal Stromal Cell Transplantations
Aizman, I et al. | Stem Cells and Development 2015 JUL
Departments of Research and Production Development, SanBio, Inc.
Human dendritic cells transfected with a human papilloma virus-18 construct display decreased mobility and upregulated cytokine production
Khaiboullina, SF et al. | International Journal of Oncology 2013 NOV
Department of Biochemistry and Molecular Biology, University of Nevada
Human Physiology, Vrije Universiteit Brussel
Department of Genetics, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University.
Protocols & Documentation
Download: Product Information Sheet
